Development and use of an internal positive control for detection of Bordetella pertussis by PCR.
نویسندگان
چکیده
An internal control of amplification was constructed by recombinant PCR to detect PCR inhibitors. This exogenous DNA was included in the reaction mixture and coamplified with the target gene. This detection was successfully applied to the diagnosis of whooping cough by amplification of a fragment of Bordetella pertussis IS481.
منابع مشابه
Rapid-cycle PCR method to detect Bordetella pertussis that fulfills all consensus recommendations for use of PCR in diagnosis of pertussis.
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 43 5 شماره
صفحات -
تاریخ انتشار 2005